WebOct 27, 2016 · Cytochalasin B is thought to bind to the barbed end of F-actin and prevent monomer addition, promoting depolymerization of F-actin. 11 In normal neutrophils, cytochalasin B amplifies opsonized zymosan-induced superoxide production. 12 However, patient neutrophils showed negligible superoxide augmentation with cytochalasin B, … WebSince cytochalasin B (CB) can convert neutrophils from phagocytic into secretory cells and facilitate respiratory burst and degranulation through disaggregation of intracellular actin network , it was then added (1 μg/mL) to magnify the reaction and the mixture was left for 3 min after activation with 0.1 μM fMLF.
Effect of cytochalasin B on intracellular free calcium
WebMay 24, 2004 · Cytochalasin B stimulation of TNF-α primed neutrophils. Neutrophils were pre-incubated with TNF-α (25 ng/ml, 20 min, 37°C) after which they were stimulated with cytochalasin B The extracellular release of superoxide anions was measured by isoluminol-amplifled chemiluminescence (CL) given as Mcpm (10 6 counts per minutes). … WebOct 2, 1980 · Use of cytochalasin B to distinguish between early and late events in neutrophil activation Biochim Biophys Acta . 1980 Oct 2;601(3):584-91. doi: … biography buck owens
Cytochalasin B triggers a novel pertussis toxin sensitive pathway …
WebSuspended in media containing calcium and magnesium, human neutrophils aggregate briefly when exposed to arachidonic acid. The magnitude and duration of this response was enhanced by the presence of increased magnesium concentration, increased magnesium plus calcium concentrations, and cytochalasin B. WebMar 1, 1988 · In both young and the old neutrophils in the presence or absence of cytochalasin B, superoxide generation increased relative to the extracellular calcium level and reached a plateau at 0.1 μmo1/L calcium. WebThe concentration of cytosolic ionized calcium, [Ca 2+] i, was measured in intact neutrophils by use of a fluorescent indicator trapped in the cytoplasm. A given rise of … biography brooke shields