WebJan 22, 2024 · The level of LDs was assayed using BODIPY 493/503 (#D3922, Thermo Fisher Scientific) according to the manufacturer's protocol. 2.5. Western blot. Western blot was performed as previously described [24]. In brief, proteins in the cell lysate or supernatants were resolved on 4%–12% Criterion XT Bis-Tris gels (#3450124, Bio-Rad, … WebNov 8, 2024 · The cells were co-stained with BODIPY™ 493/503 for lipid droplets (D3922; Thermo Fisher Scientific), LysoTracker™ Deep Red (L12492; Thermo Fisher Scientific), or MitoTracker™ (M7510; Thermo Fisher Scientific). Nuclei were blue-stained with 4′,6-diamidino-2-phenylindole (DAPI). Tumor xenograft. All animal study procedures were …
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WebSep 1, 2024 · Briefly, HepG2 cells were fixed with 4% paraformaldehyde for 10 min, then washed three times with phosphate buffer saline (PBS). After washing, cells were incubated with BODIPY 493/503 (D3922, Thermo Fisher Scientific, MA, USA) at room temperature for 1 h, followed by three washes with PBS. WebMay 14, 2024 · To analyze their lipid droplet formation for the 3D culture, the organoids were transferred to 6 super-low attachment well dishes, incubated in 0.2% BODIPY (# D3922, Thermo Fisher Scientific) in PBS for 1 hr, and then fixed in 4% paraformaldehyde (PFA) in PBS for 10 min at room temperature.
WebJul 26, 2024 · BODIPY lipid staining of 3D-cultured HOF spheroids was performed as described previously. 29, 31, 32 Briefly, 4% paraformaldehyde-fixed 3D spheroids were incubated in a mixture of 0.1% BODIPY (#D3922; Thermo Fisher Scientific), 0.1% DAPI (#D523; Doujin), and 0.1% phalloidin (#20553; Funakoshi) in PBS containing 3% bovine … WebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 …
Web493/503 (D3922; Thermo Fisher Scientific) for 30min, followed by thrice PBS washes. The IEC were observed with a laser scan- ... (Thermo Fisher Scientific) and blocked with 8% (w/v) skimmed milk in Tris buffered saline þ Tween 20 (TBST) buffer (20m M Tris–HCl, pH7·5,150mM sodiumchloride,0·1%Tween20)atroomtemper- WebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 …
WebFeb 24, 2024 · Finally, BODIPY493/503 (#D3922, Thermo Fisher Scientific, USA) staining was performed to check the lipid droplet formation. ... USA) according to the manufacturer’s instruction. SYBR Green qPCR Master Mix (#K0251, Thermo Fisher Scientific, USA) was used for quantitative real-time PCR amplification with a CFX96 real-time PCR detection …
WebOct 21, 2024 · LipidSearch™ (Thermo Fisher Scientific) was used to make lipid peak assignments in the single cells. Only peaks above a 1E4 intensity threshold and within 5 ppm of the calculated m / z value were selected. ... (#D3922, Invitrogen, USA) in PBS and incubated in the dark for 15 minutes at 37 °C with 5% CO 2. After incubation, cells were … dfw current traffic conditionsWebJul 4, 2024 · They were cultured in Dulbecco’s Modified Eagle’s medium (Thermo Fisher Scientific) media containing 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) in an incubator at 28℃. ... (D3922; Thermo Fisher Scientific) for 30 min, followed by thrice PBS washes. The IECs were observed using a laser scanning confocal microscope … dfw current delaysWebFeb 9, 2024 · To visualize the accumulation of lipids in response to OA treatment, we used boron-dipyrromethene (BODIPY) 493/503 (D3922, Thermo Fisher Scientific, MA, USA), which labels specifically ... dfw current newsWebJan 16, 2024 · Briefly, 2D or 3D 3T3-L1 cells were incubated in a mixture of 0.2% BODIPY (# D3922, Thermo Fisher Scientific, Waltham, MA, USA), Alexa Fluor 594 phalloidin (# 20553, Funakoshi, Tokyo, Japan), and DAPI (# D523, Dojindo, Kumamoto, Japan), at 1:1000 dilutions in PBS for 1 hr, and thereafter fixed in 4% paraformaldehyde in PBS for … dfw criminal lawyersWebScientist at Pharmaceutical Product Development, a part of Thermo Fisher Scientific Ashburn, Virginia, United States. 120 followers 119 … chvrches net worthWebNov 18, 2024 · DFCP1 is an ER-associated protein with two PI (3)P-binding FYVE domains. In WT cells, myc-DFCP1 formed very small punctate structures ( Figure 1E) ( Axe et al., 2008; Li et al., 2024 ). In SKO cells, the myc-DFCP1 structures appeared highly irregular and significantly enlarged ( Figure 1, E and F). dfw current radarWebMay 14, 2024 · To analyze their lipid droplet formation for the 3D culture, the organoids were transferred to 6 super-low attachment well dishes, incubated in 0.2% BODIPY (# D3922, Thermo Fisher Scientific) in ... chvrches never say die