Hif1a antibody
WebHIF1A Antibody detects endogenous levels of total HIF1A. RRID: AB_2835328 Cite Format: Affinity Biosciences Cat# AF1009, RRID:AB_2835328. Conjugate: Unconjugated. Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific). Storage: ... WebPolyclonal Antibody for studying HIF1A. Cited in 194 publications. Validated for Western Blotting. Highly specific and rigorously validated in …
Hif1a antibody
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WebRabbit anti-HIF1-alpha Antibody, Affinity Purified (alpha subunit (basic helix-loop-helix transcription factor) ); Powered by Bethyl Laboratories, Inc.; Suitable for immunohistochemistry; KR EN. Applications Products Services Support. PLA0081; All Photos (3) PLA0081. Web5 de jan. de 2024 · HIF1a Antibody (H1alpha 67) is a mouse monoclonal IgG 2b κ, cited in 183 publications, provided at 200 µg/ml. raised against amino acids 432-528 of HIF-1α of …
WebMonoclonal Antibody for studying HIF1A. Cited in 31 publications. Validated for WB, IP, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, HIF-1α (D5F3M) Mouse Monoclonal Antibody (CST #79233) is ready to ship. Web26 de fev. de 2024 · The biotin-labeled exogenous wild type or mutated HIFAL without nt 240–300 was transfected into HIFAL null MDA-MB-231 cells, and detected by FITC labeled anti-biotin antibody. Scale bars, 10 μM.
WebShowing subcellular location of HIF1A (bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8). We use cookies to enhance the usability of our website. ... Location antibody staining i. Location tagged protein i HPA000907: HeLa: 5803: N-terminal: Nucleoplasm, Plasma membrane: Nucleoplasm, Plasma membrane ... WebUnder hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular …
Web3 de out. de 2024 · After 3 washes, they were incubated with anti-Ki67 (1:25, Abcam, Cat #ab 1667), anti-CD31 antibody (1:25, BD Biosciences, Cat #550274) at 4° C. overnight and were then incubated with either Alexa 647-conjugated anti-rabbit IgG (Life Technology), or Alexa 594-conjugated anti-rabbit IgG or Alexa 488 conjugated anti-rat IgG at room …
WebThis antibody does not cross-react with ARNT or the related HIF-2 alpha. MA1-516 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, immunocytochemistry, and gel shift procedures. By Western blot, this antibody detects an ∽116 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells. smallest prime number greater than 300WebWestern blot - HIF1α Rabbit pAb (A11945) Western blot analysis of extracts of HeLa cells, using HIF1α antibody (A11945) at 1:1000 dilution.Hela cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. song of episode 1Web13 de mai. de 2024 · DNA was immunoprecipitated from the sonicated cell lysates using anti-HIF1A antibody (Abcam) and immunoprecipitates were recovered by addition of Salmon Sperm DNA/Protein A Agarose-50% Slurry. smallest prime number of 28WebDetection of human HIF1 alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells that were treated with 200 µM cobalt chloride.Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody A300-286A (lot number A300-286A-6) used for WB at 1 µg/ml and for IP at 6 µg/mg lysate.Detection: … song of exultation cluesmallest prime number that is a factor of 35WebI am doing HIF1 westerns in HIF-overexpressing mouse liver and adipose tissue using Novus antirabbit HIF1a antibody with overnight incubation. I am getting strong bands around 90kDa. I am aware that HIF theoretical molecular weight is 93kDa, but in westerns, the HIF band is usually around 120kDa according to my internet research. smallest primordial black holeWeb11 de nov. de 2015 · Abstract. Background: Hypoxia Inducible Factor (HIF) regulates a cascade of transcriptional events in response to decreased oxygenation, acting from the cellular to the physiological level. This response is evolutionarily conserved, allowing the use of zebrafish (Danio rerio) as a model for studying the hypoxic response. song of faith forsworn