WebN6-Methyladenosine ( m6A) is the most abundant modified nucleoside in mRNA. The dynamic epitranscriptomic m6A RNA modification by writers and erasers and its … WebMay 10, 2024 · To elucidate mechanistic roles of an RBP, crosslinking immunoprecipitation (CLIP) methodology can be used to capture its endogenous direct RNA targets and define the actual interaction sites. The enhanced CLIP (eCLIP) is a newly-developed method that offers several advantages over the conventional CLIPs.

Eclipsebio Expands Intellectual Property Portfolio to Support New ...

WebThe RNA m6A binding protein YTHDF2 promotes the B cell to plasma cell transition: Organism: Mus musculus: Experiment type: Methylation profiling by high throughput … WebN6-methyladenosine (m6A) is a post-transcriptional modification found in various RNA subtypes. While the presence of m6A in RNA was described decades ago, the lack of tools has made interrogating the epitranscriptomic landscape challenging (1,2). cqc rating widget

Eclipsebio Expands Intellectual Property Portfolio to Support New ...

WebMay 24, 2024 · Eclipsebio's extensive RNA genomics platform provides transcriptome-wide views of RNA interactions with RNA binding proteins (RBP-eCLIP TM), RNA modifications such as m6A (m6A-eCLIP TM), alternative transcription start site and polyadenylation site usage (End-Seq TM), microRNA-mRNA interactions (miR-eCLIP TM) and upcoming … WebWe performed m6A-eCLIP-seq to identify those Mettl3-dependent m6A RNA modifications occurring after DNA-damage. We utilized oncogene-expressing fibroblasts expressing control shRNAs or shRNAs directed against Mettl3 to acutely knockdown Mettl3, and we treated cells for 6 hours with o,2 ug/ml of doxorubicin before performing m6A-eCLIP-seq. WebBased on enhanced CLIP (eCLIP), our m6A-eCLIP (meCLIP) approach couples the improvements of eCLIP with the inclusion of an input sample and an easy-to-use computational pipeline to allow for ... distribution list not working office 365