WebbUpon uptake into a cell, sLP mCherry fluorescence has an intracellular half-life of 43 hours and is localised in CD63+ multi-lamellar bodies (lysosomal-like structures) where, owing … Webb11 dec. 2024 · We engineered breast cancer cells to stably express sLP-Cherry, a modified version of a red fluorescent protein (mCherry) containing a secretory peptide ‘s’ (ref. 5) …

Generation of neighbor-labeling cells to study intercellular

Webb58 (mCherry) containing a modified lipo-permeable Transactivator of Transcription (TATk) 59 peptide3,4 (sLP-mCherry) (Figure 1a and Extended Data Figure 1a). We engineered 4T1 60 breast cancer cells to express the sLP-mCherry protein alongside a canonical cell-retained 61 Green Fluorescent Protein (GFP), which we refer to as Labelling-4T1. WebbsLP-mCherry protein across the membrane. The number of red-labeled cells in the co- culturesystemwasabout13.9%asquantifiedbyflowcytometry(Figure3C).Asindicated upbeat female country songs

Metastatic-niche labelling reveals parenchymal cells with …

Webb12 nov. 2024 · As shown in Figure 3 B and Figure S3, red fluorescence was observed in recipient cells, confirming the transfer of sLP-mCherry protein across the membrane. The number of red-labeled cells in the co-culture system was about 13.9% as quantified by flow cytometry ( Figure 3 C). Webbslp - swedish lorry parts Swedish Lorry Parts är ett svenskt företag specialiserat på reservdelar som passar till Volvos entreprenadmaskiner, Volvo/Scanias lastbilar och … Webbcoding either wild-type mCherry–SLP-76 or mCherry–SLP-76 Y3F together with plasmids encoding tagged forms of Nck and Vav1. Western blotting analysis demonstrated that all … upbeat fiction books