WebApr 10, 2024 · Plasmid construction and Agrobacterium culture. The plant binary vector pBI121 plasmid (Fig. 1) was induced into A. tumefaciens LBA4404 and GV3101 [] via the freeze/thaw method.We selected a single colony of Agrobacterium cultured overnight in liquid YEB medium containing 50 mg/L kanamycin and 50 mg/L rifampicin (Sigma … WebGV3101::pMP90(RK); Table 2]. The pPZP plasmids7 contain the pVS1 ori (Ref. 8), which is considerably smaller than the DNA fragments from pRK2 or pRi that are necessary for plasmid replication. As a conse-quence, the basic vectors containing pVS1 replication sequences are smaller than those with the pRi or pRK2 replication region (Table 1).
Agrobacterium Competent Cells - Lifeasible
GoldBio’s GV3101 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pmp90 (pTiC58DT-DNA) harboring the gentamicin resistance. WebGV3101, followed by EHA105 (40 %), AGL1 (35 %), and MP90 (15 %). The mortality rate of cotyledons due to Agrobacterium overgrowth was the lowest with the strain GV3101. The Agrobacterium strain EHA105 was more efficient than GV3101 in the transfer of single T-DNA insertions of nptII and uidA transgenes into the tomato genome. sandy shores wellness center
Evaluation of four Agrobacterium tumefaciens strains for the …
WebThe GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid … WebApr 4, 2024 · ¥240 - GV3101(pSoup-19) 农杆菌感受态 货号 CC96303 2024-04-04 上架 WebGV3101. 种属. 根瘤农杆菌. 转化效率. 高效率 (>1×10^4 cfu/µg) 菌株用途. 植物转基因. 应用特征. 适用于拟南芥、烟草、玉米、土豆等植物的转基因操作. 菌株抗性. 利福平；庆大霉 … sandy shores state park sd